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Can a DNA Test Prove Half-Sibling Status?
Updated on August 16, 2022
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Can a DNA Test Prove Half-Sibling Status?
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More people than ever are using DNA testing to help them find relatives.

Can a DNA Test Prove Half-Sibling Status? 2

If you believe you have half-siblings that you’ve never met, genetic testing might help you identify a relationship. The test is a great place to begin your search for long-lost relatives because it helps you find DNA links.

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Do Brothers and Sisters Have the Same DNA?


DNA is unique to each individual unless he or she is an identical twin. However, DNA testing can help you identify siblings and half-siblings because you share more DNA with those people than with people not related to you. But simply sharing DNA with someone doesn’t necessarily mean that person is a sibling.

Some sibling DNA, especially when people are half-siblings, is the same as more distant relatives, such as cousins or aunts and uncles.

To understand this better, it helps to have a clearer picture of how DNA works.

DNA is packed into cell units called chromosomes. Each cell in your body contains two copies chromosomes – one from your mother and one from your father. Most of us have 46 total chromosomes in the body.

Scientists numbered chromosomes 1 through 23 to make them easier to locate and understand. The 23rd pair is significant because it establishes biological sex.

Females (XX) inherit X chromosomes from both parents.

Males (XY) inherit a Y chromosome instead of an X from their fathers.

How much DNA do brothers and sisters share?

Biological siblings with the same parents share about 50% of their DNA.

You got about half of your DNA from your mother and your brother or sister got half of his or her DNA from the same mother.

This means you and your full biological sibling share half of 50% of your mother’s DNA. This is 25%. If they share the same father, they inherited about 25% of their father’s DNA and share 50% total – half from mom and half from dad.

Can two brothers have the same DNA? How Much DNA Do Brothers Share?

Yes, absolutely, but not exactly the same.

Siblings have some of the same DNA and more of the same DNA than more distant relations. The same is true for sisters and sister and brother pairs. All share the same DNA, but it varies to some degree in the total amount.

It can be challenging to figure out the percentages and imagine what exactly is contributed by a sibling pair’s mother and father.

It helps to imagine the process of passing on DNA with a deck of cards in mind. Shuffle the 52 cards in the deck and lay 26 of them face up. The cards you see represent half of your mother’s DNA that you inherited.

Now put the entire deck back together, reshuffle, and lay 26 cards face up again to represent your full sibling’s DNA. Chances are good you aren’t going to get the exact same 26 cards, but some of them will be the same.

Both you and your sibling each got 26 out of your mother’s 52 cards/DNA, but you didn’t get the same 26.

To determine the total amount of DNA shared between you and a full sibling, you’d repeat this exercise for your father’s DNA. But if you are only half-siblings, you’d only do it once, for your mother or your father – whichever parent you share.

The cards that match when you layout 26 for each of you represent about 25% - the amount of DNA you share with a half-sibling contributed by your shared parent.

Now, keep in mind, these percentages vary from person to person. You aren’t going to get a match of 13 cards each time, though it will happen occasionally. The 26 chromosomes we get from each parent are random.

Theoretically, you could have them all match or have none of them match with a sibling. So while the “rule” is siblings share about 50% of their DNA and half-siblings share about 25%, they don’t have to share any at all.

We inherit approximately 50% of our DNA from our mothers and 50% from our fathers, but that’s where “exact” percentages end.

So not only can a brother and sister have different DNA, most do, but it’s in random varying amounts.

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Can a DNA Test prove Half-Siblings?

DNA testing can help show the potential for a sibling relationship, but it won’t prove it with certainty.

As a matter of fact, it’s as likely that a person is your half-sibling as it is that they are your cousin. Half-siblings also have a similar genetic presentation as grandparents and aunts and uncles.

The best DNA testing can narrow down the relationship better than basic tests, so if you are relying on an at-home test to find a half-sibling, you’ll likely need to dig deeper to prove the relationship.

At-home tests are a great place to start, and then you can follow up by adding known family members and conducting additional research.

How much DNA do half siblings share?

Half-siblings share about a quarter (25%) of their DNA. Depending on how it is passed down from parents to children, some half-siblings will have more in common while others have less.

This difference in shared DNA is more prominent when comparing half-sisters to sister-brother sibling pairs. Half-sisters who share a father will have more DNA in common than a half-brother and half-sister because of how the X and Y chromosomes are passed down.

Biological males carry an X and Y chromosome in their DNA while biological females carry two X's. This means fathers always pass the Y chromosome to their sons and X to their daughters.

Consequently, half-sisters who share a father will have one X chromosome that they share entirely, that their brother doesn’t have. Half-sisters will always have more DNA in common than they do with a half-brother.


  • For clarity, a half-sibling is a sibling with whom you share a single parent. If your sibling is a child from your step-parent's relationship with someone besides your parent, you will not be genetically related.
  • It is also worth noting that DNA ranges found in persons who share close family relationships can vary and overlap. In simple terms, different degrees of relationships may share the same amounts of DNA. This means your DNA test may misrepresent your half-sibling as a grandchild, niece, aunt, or uncle.

How to find a relative using DNA

After you take an Autosomal DNA test (A DNA test that examines your autosomal chromosomes, which contain DNA segments you share with everyone to whom you are related), your testing company will compare your results to others in its database.

If it finds significant similarities to other people who have had the genetic test, you will see them in your list of matches.

The company determines the likelihood that you're a relative to another test-taker based on the amount of shared DNA.

If your ancestry is in a well-studied region like Europe, you may have thousands of matches. While most will be only distantly related to you, a few will likely be close matches. 

Calculating the probability of finding a sibling

A standard sibling test compares two possible outcomes, known as hypotheses.

For instance, two individuals who are aware they share the same mother might ask a DNA testing company to verify that they share the same father as well.

In this scenario, the DNA testing company evaluates hypothesis 1 (Jane and Joe are full siblings) against hypothesis 2 (Jane and Joe are half-siblings sharing the same mother but with different fathers).

The usual outcome is a probability of about 99% of hypothesis 1 "Jane and Joe" are full siblings, and 0.07 for hypothesis 2, Jane and Joe are half-siblings sharing the same mother, but having different fathers.

Scenario 2: half siblingship

If two people are aware that they have different mothers but are uncertain whether they share a father, the DNA testing company will perform half a sibling test.

First, analysts will test hypothesis one.

Jane and Joe are half-siblings, sharing a father but having different mothers, against hypothesis 2, Jane and Joe are unrelated, i.e., they do not share a father or mother.

If Jane and Joe are unrelated, the usual outcome will be a probability of 1.2% for hypothesis 1 and 90% for hypothesis 2.

If you do want to get a test to start taking steps to determine sibling status, we recommend one of the following kits:

This image has an empty alt attribute; its file name is 81p1yWPlyAS._AC_SL1500_-791x1024.jpg

My Forever DNA Sibling DNA Test - The DNA Test Specifically for Siblings

We recommend myforever DNA Complete Home Sibling DNA test because it is one of the only DNA tests that is made specifically for testing sibling status.

Know Your DNA Reviews

The Best DNA Test

Looking for a DNA test that's accurate and can tell you about your health and heritage?

Content Contributor
Joel is a writer that loves exploring DNA, at-home health, and optimizing my health. He believes that the democratization of home health is in its early stages.
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